Recent years, ex vivo cultured limbal stem cells for transplantation in the treatment of limbal stem cell deficiency (LSCD) in clinical applications has achieved good therapeutic effect. In order to obtain stem cells of limbus of cornea to more people from the carrier of stem cell cultivation system experts has done a lot of explorations. It has been found that ex vivo cultured the stem cells is very easy to differentiate and the limited number of stem cells has made the research on the preparation and mechanism of the feeder layer become a hotspot at domestic and international, this article will discuss the feeder layers of the preparation, mechanism and the latest research progresses. (Int Rev Ophthalmol, 2016, 40: 348-352)