Abstract: Limbal niche cells (LNCs) are a kind of mesenchymal stem cells isolated from limbal stem cells microenvironment. These niche cells display a phenotype typical of vascular endothelial progenitor cells and mesenchymal stem cells. LNCs support limbal epithelial stem cells viability and exhibit a potential to differentiate to multiple cell types such as vascular endothelial cells, corneal epithelial cells, corneal stromal cells and so on. Therefore, they can prevent or treat limbal stem cell deficiency caused by alkaline burns in animal experiments. LNCs have also shown the ability to remodel pathological stromal tissue, suppressing inflammation and neovascularization and restoring transparency. All these abilities give LNCs the potential for allograft or even autologous stem cells treatment for corneal wound healing and other corneal diseases.