Abstract: The pathogenesis of cataract is relatively complex, lack of effective drug treatment, and postoperative visual quality cannot be restored to normal state. With the application of stem cell technology in ophthalmology, researchers have used stem cells from various sources to successfully induce the formation of humanoid lenses in vitro and reproduce the microscopic three-dimensional structure of some of their unique functions. These lens organoids are used to build disease models in vitro, study the mechanisms and inductions of related diseases, screen drugs and even regenerate alternative treatments. It provides more possibilities for preventing and treating cataracts. This paper reviews the research progress of lens organoids, introduces the breakthrough research methods, summarizes their clinical applications in mechanism research, drug screening and regenerative replacement therapy, and puts forward personal prospects in the direction of related research and cultivation and the construction of 3D lens or whole eye structure in cooperation with multi-bioengineering. (Int Rev Ophthalmol, 2024, 48:  401-406)

Key words: cataract, organoids, disease model