Ophthalmology in China ›› 2014, Vol. 23 ›› Issue (6): 384-387.doi: 10.13281/j.cnki.issn.1004-4469.2014.06.007

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Drug resistance analysis of corneal pseudomonas aeruginosa infection in 2007-2013

ZHANG Yang, WANG Zhi-qun, SUN Xu-guang   

  1. Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100005, China
  • Received:2014-08-05 Online:2014-11-25 Published:2014-12-01
  • Contact: SUN Xu-guang, Email: Sunxg1955@163.com E-mail:Sunxg1955@163.com

Abstract: Objective To analyze the drug sensitivity and resistance of corneal pseudomonas aeruginosa from Beijing Tongren Hospital in 2007-2013 in order to provide guidance for clinical drug treatment. Design Experimental study. Participants 100 corneal pseudomonas aeruginosa in Beijing Tongren Hospital were isolated from ophthalmological specimens for microbiological examination. Methods  Kirby-Bauer disk diffusion method in vitro is done by spreading bacterial diluents containing isolated and cultured corneal pseudomonas aeruginosa on Mueller-Hinton (M-H) agar plate and pasting drug disks. Sensitivity is interpreted according to inhibition zone diameter and standards from Clinical Laboratory Standards Institute (CLSI). Based on principles of bacterial biochemical tests, ATB Expression can semi-automatically identify bacteria. WHONET 5.6 and SPSS 16.0 are applied for data processing. Main Outcome Measures Drug sensitivity and resistance. Results Sensitive and resistance rates of 9 drugs were: amikacin 93.8% and 3.1%, tobramycin 86.9% and 12.1%, gentamicin 67.3% and 17.3%, ciprofloxacin 89.1% and 8.7%, levofloxacin 88.7% and 10.3%, levofloxacin 86.9% and 11.1%, gatifloxacin 87.4% and 11.5%, moxifloxacin 41.7% and 36.5%, ceftazidime 84.8% and 8.1%. 3% of the isolates were resistant to all drugs tested. Conclusion Corneal pseudomonas aeruginosa is most sensitive and least resistant to amikacin. Sensitive rates of ciprofloxacin, levofloxacin, ofloxacin, gatifloxacin and tobramycin all exceed 85%. Clinical application of combination drug therapy should be considered. Corneal pseudomonas aeruginosa is highly resistant to moxifloxacin which application should be cautious.

Key words: pseudomonas aeruginosa keratitis, sensitive rate, resistance rate