Abstract: Objective To investigate the infection-related factors, ocular manifestations and transmission routes of coronavirus disease 2019 (COVID-19) in the hospitals with eye professionals diagnosed symptomatic COVID-19 in Wuhan. Design Cross-sectional case-control questionnaire survey. Participants A total of 28 eye professionals who contracted COVID-19 in Wuhan, and 96 non-infected control subjects who were randomly selected with a 1 to 3-4 ratio within each case’s own hospital from February 26, 2020 to March 20, 2020. Method The "questionnaire network" was used to design the questionnaire and the data were downloaded from the background of the "questionnaire network". Main Outcome Measures The general information of eye professionals, their 2019-novel corona virus (2019-nCoV) PCR results, ocular symptoms, and treatments. Results A total of 28 eye professionals were diagnosed COVID-19 from January 16, 2020 to February 29, 2020 with an average age of 41.9±11.7(29-70) years old, from 10 general and ophthalmic hospitals in Wuhan. The control group included 96 eye professionals with an average age of 35.1±5.5(26-52) years old. There were 19 female subjects(67.9%), 14 doctors (50%), 12 nurses (42.9%), 2 technicians (7.1%) in the case group. 19 subjects(67.9%) worked in ophthalmology department and 9 subjects(32.1%) worked in anti-epidemic front line, which was no significant different compared with the control group. There are 8 severe cases(28.6%) (including 3 deaths and 1 critical case) in the case group. Reverse-transcriptase polymerase chain reaction (RT-PCR) of nasopharyngeal swabs were positive for 2019-nCoV in 15 cases(53.6%). 25.7% of infected medical workers found the working environment poorly ventilated. The questionnaire was completed in 26 cases (92.9% response rate) and 96 controls (100% response rate). There was a higher rate of concurrent ocular symptoms in 11 cases(42.3%) with COVID-19 than those (15.6%) in the control group (P=0.003). Among them, 10 cases(90.9%) with eye itching significantly higher than the control group (73.3%) (P=0.001), 4 cases (36.4%) with tearing significantly higher than the control group (13.3%) (P=0.02), 7 cases (63.6%) with foreign body sensation, 2 cases(18.2%) with eye redness, and 2 cases (18.2%) of blurred vision in case group. Among 4 subjects tested for 2019-nCoV PCR of conjunctival swabs, 1 subject (3.6% of total 28) was positive. Ocular symptoms were found in 9 cases(45%) of 20 mild cases and in 2 cases(33.3%) of 6 severe cases. There was no significant correlation between COVID-19 severity and ocular symptoms. Conclusion This questionnaire survey found that 42.3% of COVID-19 patients had ocular symptoms. Conjunctival sac 2019-nCoV test existed positive confirmed that there was 2019-nCoV in conjunctival sac. Eye professionals who are at a high risk of COVID-19 infection, need to pay attention to conjunctivitis symptoms patients and strengthen ocular medical protection to prevent the nosocomial spread of COVID-19. （Ophthalmol CHN, 2020, 29: 332-338）

Key words: novel-corona virus disease 2019, eye professionals, questionnaires, personal protective equipment, ocular manifestations