Abstract:  Objective  To explore the preliminary efficacy of preoperative process improvement on intravitreal drug injection. Design Prospective comparative case series. Participants 500 patients undergoing intravitreal injection surgery in ophthalmic daytime operation room of Beijing Tongren Hospital. Method The preoperative process of intravitreal injection procedures was improved (study group, n=260 cases), including adjusting the order of application items in the surgical appointment form, adding color blocks to distinguish different injectable drugs, marking the surgical eye with multiple stickers with different color and fonts, and adjusting the frequency and timing of topical anesthetics. Traditional preoperative procedure (control group, n=240 cases) was compared with study group. Main Outcome Measures The time for checking surgical appointment form, the satisfaction of doctors and nurses on the improved surgical appointment form, the proportion of difficult surgical checking due to various reasons, and the intraoperative pain score of patients. Result The time for checking surgical appointment form in control group and study group were 5.00±0.51 min and 2.00±0.49 min, respectively (t=64.132, P<0.001). The proportion of satisfied with the surgical appointment form in surgeons and itinerant nurses was 100% in the study group and 20% in the control group. The proportion of unclear eye-marker caused by eyewash was 41.7% in the control group and 0.0% in the study group, respectively (χ2=135.41, P<0.001). The proportion of the huck towel covering the surgical eye-marker was 100% in the control group and 0% in the study group, respectively (χ2=192.4 , P<0.001). Intraoperative pain score in study group was 2.14±0.15, less than in control group (2.82±0.54) (t=8.708, P<0.001).  Conclusion Preoperative process improvement on intravitreal drug injection can help to increase the efficiency of intravitreal injection surgery, and increase medical staff satisfaction, and decrease the intraoperative pain of intravitreal injection. (Ophthalmol CHN, 2022, 31:  463-466)