Abstract: Objective To evaluate the safety and effectiveness of trabeculectomy combined with anterior chamber proliferative membrane interception (AC-PMI) on neovascular glaucoma (NVG).  Design Retrospective case series. Participants Twenty-eight patients (31 eyes) with NVG who underwent trabeculectomy combined with AC-PMI in the Eye Hospital of Wenzhou Medical University. Methods Medical records of patients were reviewed and followed up at one day, one week, month 1, 3, 6, and 12-momth postoperatively. Repeated measured ANOVA was applied to compare pre- and postoperative intraocular pressure (IOP) and number of anti-glaucoma medication. Kaplan-Meier survival-curve analysis was used to evaluate cumulative probability of surgery success.  Main Outcome Measures IOP, number of anti-glaucoma medication, surgery-related complications and success rate of operation. Results The mean follow-up was 11.3±3.8 months. The mean IOP decreased from (51.3±8.3) mmHg on 2(2, 3) medications before surgery to (14.2±5.1) mmHg (P<0.001) on 0(0, 0) medications (P=0.002) at 12 months post-operation, respectively. The cumulative complete success rate and qualified success rate were 72.6% and 87.3% at 12 months post-operation, respectively. The incidence of hypotension, hyphema, shallow anterior chamber, choroidal detachment and descemet’s membrane fold all were 3.2% (1/31). Conclusions Trabeculectomy combined with AC-PMI can effectively reduce and stabilize IOP in NVG patients, and is an alternative combined surgical approach for NVG. (Ophthalmol CHN, 2023, 32: 21-26)