Ophthalmology in China ›› 2012, Vol. 21 ›› Issue (4): 256-260.

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Stromal cell-derived factor-1 promotes axonal regeneration of retinal ganglion cells in retinal explants

CEN Ling-ping, LIANG Jia-jian, ZHANG Ming-zhi.   

  1. Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, China
  • Received:2012-05-08 Online:2012-07-25 Published:2012-07-27
  • Contact: 岑令平,Email:cenlp@gmail.com

Abstract: 【Abstract】 Objective To investigate whether stromal cell-derived factor-1 (SDF-1) has promoting effects on axonal regeneration in retinal explant. Design Experimental study. Participants Fischer 344 rats retina. Methods Optic nerve of rats were crushed 5 days before retinal explant culture, and culture plate was coated with poly-lysine and laminin before culture. Retinas were dissected out from eye cups and cut into 8 pieces followed by sticking onto the coated wells with retinal ganglion cell layer downward. Retinal pieces were incubated in neurobasal-A/B27 for 7 days before examination under an inverted fluorescent microscope. Explants were assigned to the following groups: control, SDF-1 groups with 20 ng/ml, 70 ng/ml, 200 ng/ml, 500ng/ml, 1000 ng/ml, and SDF-1 plus AMD3100 group. Main Outcome Measures Number and average length of regenerating axons. Results Average umber and length of the regenerating axons in control group were 17/explant and 1mm respectively. Promoting effects of SDF-1 was positively correlated to its concentrations. SDF-1 with middle and high concentration enhanced the axonal regeneration by 2-3 folds. The promoting effect of SDf-1 was reduced by half when adding the receptor inhibitor AMD3100. Conclusions SDF-1 has promoting effects on axonal regeneration of retinal ganglion cells in retinal explants and has dose-response. (Ophthalmol CHN, 2012, 21: 256-260)

Key words: retinal explant, stromal derived factor-1, axonal regeneration