Abstract:

【Abstract】Objective To study the feasibility of porcine fibrin sealant kit as substitution of intraocular filling for closing the retinal hole. Design Experimental study. Participants 45 Qing-zilan rabbits (45 eyes). Methods Vitrectomy combined with puncture in retina and injecting balanced salt solution underneath the retina was performed for establishing hegmatogenous retinal detachment model in each eye of 45 Qing-zilan rabbits. Retinal reattachment surgery and laser photocoagulation line around the hole were performed in all groups. Then porcine fibrin sealant kit (Guangzhou Beixiu Corporation) was used to close retinal holes and perfusion fluid was filled in vitreous cavity in the fibrin glue group (14 eyes), 30% SF6 was filled in vitreous cavity in the gas group (14 eyes), and single perfusion fluid was filled in vitreous cavity in control group (14 eyes). Indirect ophthalmoscopy, fundus photography, B ultrasonography, OCT, and histopathologic examinations were preformed at 1week, and 4 weeks postoperatively. Main Outcome Measures Rate of retinal reattachment. Results On the 4th week after reattachment surgery, 12/14 eyes (85.7%) retinal reattached in fibrin glue group, while 13/14 eyes (92.9%) retinal reattached in gas group. There was no significant difference statistically between these two groups (P =1.00). However, only 5/14 eyes (35.7%) retinal reattached in the control group. Compared with the former two groups, there were significantly statistical differences (P=0.009, 0.036). Conclusions The result of experiment in rabbits shows that porcine fibrin sealant kit can replace intraocular fillings in closing retinal holes temporarily. (Ophthalmol CHN, 2015, 24: 254-257)