Studies on effects and meaning of micropulse subthreshold 577nm laser photocoagulation on mRNA expression of VEGF,PEDF and TGF-β2 in neural retina of rabbit

doi:10.13281/j.cnki.issn.1004-4469.2017.02.010

Abstract

Abstract:

Objective To investigate the effects of micropulse subthreshold 577nm laser photocoagulation on mRNA expression level of VEGF, PEDF and TGF-β2 in neural retina of rabbit. Design Experimental animal study. Participants 14 healthy adult livor blue rabbits. Methods Rabbits were randomly divided into 2 groups: blank control group (n=2) and experimental group (n=12). Micropulsed subthreshold photocoagulation was done using 577nm laser. The morphological changes of retina were observed at 3 days after photocoagulation under microscopy using HE staining of histopathological sections． The mRNA expression level of VEGF, PEDF and TGF-β2 in neural retina were analyzed by RT-PCR at 3 days, 7 days and 14 days after photocoagulation． Main Outcome Measures The mRNA expression level of VEGF, PEDF and TGF-β2 in each time point. Result There was neither visible laser lesion under ophthalmoscope nor retinal tissue damage observed by microscope observation in retina of all experimental rabbits after photocoagulation. RT-PCR results showed that the mRNA expression level of VEGF, PEDF and TGF-β2 increased after laser photocoagulation. The VEGF mRNA expression level was 2.1420±0.1217, 2.5207±0.2568 and 1.5525±0.2118 on day 3, day 7 and day 14 after photocoagulation respectively. There was no statistically significant difference between day 3 and 7 (P=0.099), while the difference between day 7 and 14 showed statistical significance (P=0.029, 0.005). The PEDF mRNA expression level was 3.2623±0.1442, 6.4050±0.1117 and 4.2976±0.2813 on day 3, 7 and 14 respectively. There was statistically significant difference between day 3, 7 and 14 (P=0.0003, 0.002, 0.0002). At the same time points, the expression level of TGF-β2 mRNA was 2.7054±0.1571, 2.5747±0.0858 and 2.3128±0.2742 respectively, with no statistically significant difference between each time point (P=0.155). Conclusion Micropulsed 577nm laser photocoagulation can upregulate the VEGF, PEDF and TGF-β2 expression in rabbit neural retina, which may be related to the mechanism of treating macular edema. (Ophthalmol CHN, 2017, 26: 112-116)

Key words: micropulsed laser photocoagulation, retina, vascular endothelial growth factor, pigment epithelium derived factor, transforming growth factor-β； rabbit

SUN Di, MA Dan-dan, GUO Yin-hua, XIAO Yuan-yuan, LI Liao-qing, LI Yi-bin. Studies on effects and meaning of micropulse subthreshold 577nm laser photocoagulation on mRNA expression of VEGF,PEDF and TGF-β2 in neural retina of rabbit[J]. Ophthalmology in China, doi: 10.13281/j.cnki.issn.1004-4469.2017.02.010.

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Studies on effects and meaning of micropulse subthreshold 577nm laser photocoagulation on mRNA expression of VEGF,PEDF and TGF-β2 in neural retina of rabbit

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