Abstract:  Objetive To investigate the effect of PDLIM1 on autophagy and vascular formation of rats retinal capillary endothelial cells (RCECs) with high glucose culture. Design Experiment study. Participants Rats retinal capillary endothelial cells. Methods The RCECs were randomly divided into normal glucose (NG), high glucose (HG), high glucose+negative control (HG+NC) and HG+ad-rPDLIM1 groups. Tubule formation test, scratch test were used to determine the ability of cell tube formation and proliferation.The protein and mRNA expression levels of PDLIM1, LC3 and Beclin-1 were verified by Western blotting and RT-qPCR. Main Outcome Measures The number of cell tubes, the amount of cell migration and the protein and mRNA levels of LC3 and Beclin-1 antibodies. Results The number of tube formation and migration of cells in the HG group were higher than those in NG group, and the expression of PDLIM1 decreased in the HG group, while the expression of LC3 and Beclin-1 increased. After overexpression of PDLIM1, compared with the HG group, the number of tube formation (2.33±1.15) and cell migration (12.33±1.08)% significantly decreased (t=9.59, P<0.001; t=22.39, P<0.001), and the expression of LC3 and Beclin-1 significantly decreased (t=22.39, P<0.001; t=11.1, P<0.001). Conclusions PDLIM1 may inhibit retinal neovascularization in rats through downregulating autophagy. (Ophthalmol CHN, 2024, 33: 290-294)

Key words: PDLIM1, autophagy, diabetic retinopathy