577 nm激光微脉冲光凝对兔神经视网膜层VEGF、PEDF及TGF-β2 mRNA表达的影响及意义

doi:10.13281/j.cnki.issn.1004-4469.2017.02.010

眼科

577 nm激光微脉冲光凝对兔神经视网膜层VEGF、PEDF及TGF-β2 mRNA表达的影响及意义

孙镝马丹丹郭银华肖媛媛李辽青李毅斌

100005 首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科研究所眼科学与视觉科学北京市重点实验室

收稿日期:2017-01-19 出版日期:2017-03-25 发布日期:2017-03-29
通讯作者: 李毅斌，Email：yibinlee@yahoo.com
基金资助:
北京市眼科研究所重点学科引领计划（201507）

Studies on effects and meaning of micropulse subthreshold 577nm laser photocoagulation on mRNA expression of VEGF,PEDF and TGF-β2 in neural retina of rabbit

SUN Di, MA Dan-dan, GUO Yin-hua, XIAO Yuan-yuan, LI Liao-qing, LI Yi-bin

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100005, China

Received:2017-01-19 Online:2017-03-25 Published:2017-03-29
Contact: LI Yi-bin, Email : yibinlee@yahoo.com

摘要/Abstract

摘要：

目的研究577 nm激光微脉冲光凝对兔眼神经视网膜层VEGF、PEDF及TGF-β2 mRNA表达的影响及意义。设计动物实验研究。研究对象 健康成年青紫蓝兔14只。方法实验兔随机分为2组：空白对照组（2只）及实验组（12只）。采用577 nm激光对实验组兔眼视网膜进行阈值下微脉冲光凝。石蜡切片HE染色观察光凝后3天（3只）视网膜组织形态学改变。实时荧光定量PCR检测光凝后3天（3只）、7天（3只）和14天（3只）时神经视网膜中VEGF、PEDF及TGF-β2的mRNA表达量。主要指标 VEGF、PEDF及TGF-β2的mRNA在不同时间点的表达量。结果光凝后所有实验兔视网膜均未见激光斑形成，组织学观察未见典型视网膜组织损伤。光凝后3天、7天及14天神经视网膜中VEGF、PEDF及TGF-β2的mRNA表达量与对照相比均升高。光凝后3天、7天及14天，VEGF在的表达量分别为2.1420±0.1217、2.5207±0.2568和1.5525±0.2118，其中3天与7天的表达量差异无统计学意义（P=0.099），而14天与3天和7天比较明显减少（P=0.029、0.005）。PEDF表达量分别为3.2623±0.1442、6.4050±0.1117和4.2976±0.2813，各时间点表达量差异均有统计学意义（P=0.0003、0.002、0.0002）。TGF-β2表达量分别为2.7054±0.1571、2.5747±0.0858和2.3128±0.2742，各时间点的表达量差异均无统计学意义（P=0.155）。结论 577nm激光微脉冲光凝可上调正常兔眼神经视网膜层VEGF、PEDF及TGF-β2表达，可能与其治疗黄斑水肿的机制相关。（眼科， 2017, 26： 112-116）

关键词: 微脉冲激光光凝, 视网膜, 血管内皮生长因子, 色素上皮衍生因子, 转化生长因子-&beta, 兔

Abstract:

Objective To investigate the effects of micropulse subthreshold 577nm laser photocoagulation on mRNA expression level of VEGF, PEDF and TGF-β2 in neural retina of rabbit. Design Experimental animal study. Participants 14 healthy adult livor blue rabbits. Methods Rabbits were randomly divided into 2 groups: blank control group (n=2) and experimental group (n=12). Micropulsed subthreshold photocoagulation was done using 577nm laser. The morphological changes of retina were observed at 3 days after photocoagulation under microscopy using HE staining of histopathological sections． The mRNA expression level of VEGF, PEDF and TGF-β2 in neural retina were analyzed by RT-PCR at 3 days, 7 days and 14 days after photocoagulation． Main Outcome Measures The mRNA expression level of VEGF, PEDF and TGF-β2 in each time point. Result There was neither visible laser lesion under ophthalmoscope nor retinal tissue damage observed by microscope observation in retina of all experimental rabbits after photocoagulation. RT-PCR results showed that the mRNA expression level of VEGF, PEDF and TGF-β2 increased after laser photocoagulation. The VEGF mRNA expression level was 2.1420±0.1217, 2.5207±0.2568 and 1.5525±0.2118 on day 3, day 7 and day 14 after photocoagulation respectively. There was no statistically significant difference between day 3 and 7 (P=0.099), while the difference between day 7 and 14 showed statistical significance (P=0.029, 0.005). The PEDF mRNA expression level was 3.2623±0.1442, 6.4050±0.1117 and 4.2976±0.2813 on day 3, 7 and 14 respectively. There was statistically significant difference between day 3, 7 and 14 (P=0.0003, 0.002, 0.0002). At the same time points, the expression level of TGF-β2 mRNA was 2.7054±0.1571, 2.5747±0.0858 and 2.3128±0.2742 respectively, with no statistically significant difference between each time point (P=0.155). Conclusion Micropulsed 577nm laser photocoagulation can upregulate the VEGF, PEDF and TGF-β2 expression in rabbit neural retina, which may be related to the mechanism of treating macular edema. (Ophthalmol CHN, 2017, 26: 112-116)

Key words: micropulsed laser photocoagulation, retina, vascular endothelial growth factor, pigment epithelium derived factor, transforming growth factor-β； rabbit

孙镝马丹丹郭银华肖媛媛李辽青李毅斌. 577 nm激光微脉冲光凝对兔神经视网膜层VEGF、PEDF及TGF-β2 mRNA表达的影响及意义[J]. 眼科, doi: 10.13281/j.cnki.issn.1004-4469.2017.02.010.

SUN Di, MA Dan-dan, GUO Yin-hua, XIAO Yuan-yuan, LI Liao-qing, LI Yi-bin. Studies on effects and meaning of micropulse subthreshold 577nm laser photocoagulation on mRNA expression of VEGF,PEDF and TGF-β2 in neural retina of rabbit[J]. Ophthalmology in China, doi: 10.13281/j.cnki.issn.1004-4469.2017.02.010.

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577 nm激光微脉冲光凝对兔神经视网膜层VEGF、PEDF及TGF-β2 mRNA表达的影响及意义

Studies on effects and meaning of micropulse subthreshold 577nm laser photocoagulation on mRNA expression of VEGF,PEDF and TGF-β2 in neural retina of rabbit

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