Abstract: Objective To investigate the perceptual eye position and gaze stability of the children with amblyopia under dichoptic vision condition. Design Prospective case series. Participants 90 mild, moderate, severe amblyopic children with normal eye position, each include 30 cases. Method The main test method was the computer based visual perceptual test software, each amblyopic child must take the visual perceptual test and gaze stability test. Main Outcome Measures The deviation pixels of perception eye position and the gaze stability. Results When the target size was 3 degree, the mean horizontal eye position of mild, moderate, severe amblyopic children was 12.70±9.57, 23.33±21.03, 53.79±58.92 pixels respectively (H=14.765, P=0.001); the mean vertical eye position was 4.93±5.21, 9.23±9.03, 20.48±23.75 pixels respectively (H=13.569，P=0.001). When the target size was 1 degree, the mean horizontal eye position of mild, moderate, severe amblyopic children was 18.67±16.19, 33.27±27.95, 81.61±76.02 pixels respectively (H=17.941，P=0.000); the mean vertical eye position was 4.27±5.46, 7.50±10.52, 20.75±22.99 pixels respectively (H=19.927，P=0.000). The mean gaze stability deviation of mild, moderate, severe amblyopic children was 4.77±3.18, 7.23±5.54, 20.44±22.99 pixels respectively (H=29.944，P=0.000). Conclusion The deviation pixels of perception eye position and the gaze stability were abnormal in the amblyopic children with normal eye position. The more severe the degree of amblyopia, the more serious the abnormality. The treatment of amblyopia should focus on the improvement of the binocular vision.

Key words: amblyopia, visual perception, eye position；gaze stability