增生性糖尿病视网膜病变患者房水及血清IL-1β与IL-1Ra含量分析

doi:10.13281/j.cnki.issn.1004-4469.2021.06.008

眼科 ›› 2021, Vol. 30 ›› Issue (6): 449-452.doi: 10.13281/j.cnki.issn.1004-4469.2021.06.008

增生性糖尿病视网膜病变患者房水及血清IL-1β与IL-1Ra含量分析

林仲¹ 牛彤彤² 杜林² 张晨曦² 张绍丹¹ 王海林²

¹温州医科大学附属眼视光医院 325027； ²沈阳市第四人民医院眼科沈阳市眼病中心重点实验室110031

收稿日期:2020-10-30 出版日期:2021-11-25 发布日期:2021-12-10
通讯作者: 牛彤彤，Email：tongtongniu968@hotmail.com E-mail:tongtongniu968@hotmail.com
基金资助:
沈阳市重点实验室建设项目（F15-160-1-00）；沈阳市重点科技研发计划项目（17-230-9-03）；温州市基础性医疗卫生科技项目（Y2020364）

Levels of IL-1β and IL-1Ra in aqueous humor and serum of patients with proliferative diabetic retinopathy

Lin Zhong¹, Niu Tongtong², Du lin², Zhang Chenxⁱ², Zhang Shaodan¹, Wang Hailin²

¹The Eye Hospital, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325027, China; ² Department of Ophthalmology, The Fourth People’s Hospital of Shenyang, Key Laboratory of Eye Diseases of Shenyang, Shenyang 110031, China

Received:2020-10-30 Online:2021-11-25 Published:2021-12-10
Contact: Niu Tongtong, Email: tongtongniu968@hotmail.com E-mail:tongtongniu968@hotmail.com
Supported by:
Shenyang Key Lab Construction Program (F15-160-1-00); Key Scientific and Technological Research and Development Projects of Shenyang (17-230-9-03); Wenzhou Basic Medical and Health Technology Project (Y2020364)

摘要/Abstract

摘要： 目的探索增生性糖尿病视网膜病变（proliferative diabetic retinopathy，PDR）患者房水及血清白介素1β（IL-1β）、白介素1受体拮抗剂（IL-1Ra）与血管内皮生长因子（VEGF）水平的变化及其意义。设计前瞻性比较性病例系列。研究对象 36例PDR患者与26例非糖尿病白内障患者（对照组）。方法采集患者外周血，并于白内障摘除术或玻璃体切除术中抽取未稀释的房水。采用免疫磁珠多重因子检测方法分析房水与血清中IL-1β、IL-1Ra、VEGF含量。比较两组细胞因子含量的差异。主要指标 房水与血清中IL-1β、IL-1Ra、VEGF含量。结果 PDR组房水中的IL-1β（6.7±4.3 pg/ml）和IL-1Ra（657.9 ± 445.6 pg/ml）含量均高于对照组（3.3±3.2 pg/ml和300.8 ± 368.0 pg/ml，P均=0.001）；血清中的IL-1β（7.6±4.6 pg/ml）和IL-1Ra（437.8±270.2 pg/ml）含量亦高于对照组（4.9±3.7 pg/ml和279.2±226.7 pg/ml，P均=0.02）。两组房水及血清中VEGF含量差异均无统计学意义。两组房水或血清中IL-1β含量均与各自同一样本中IL-1Ra的含量呈高度正相关。PDR组房水与血清中的IL-1β（r=0.50，P=0.003）、房水与血清中的IL-1Ra（r=0.66，P<0.001）含量均为正相关，但房水与血清中的VEGF含量之间无明显相关（r=-0.06，P=0.72）。结论 PDR患者房水与血清中炎症因子升高的同时伴随抗炎因子的代偿性增高。针对炎症反应的调控可能为DR的治疗开拓新的思路。血清中炎症因子与抗炎因子水平可能作为DR发生的生物标志物。（眼科，2021, 30： 449-452）

关键词: 增生性糖尿病视网膜病变, VEGF, IL-1β, IL-1Ra

Abstract: Objective To explore the level changes of interleukin-1β (IL-1β), interleukin-1 receptor antagonist (IL-1Ra), and vascular endothelial growth factor (VEGF) in aqueous humor and serum of patients with proliferative diabetic retinopathy (PDR). Design Prospective comparative case seiries. Participants 36 PDR patients and 26 cataract patients without diabetes (control group) were enrolled. Methods The peripheral blood and undiluted aqueous humor (operatively) of all patients were collected. The cytokine concentrations of aqueous humor and serum were analyzed by immune multiplex bead-based assay. The general information and cytokine concentration were compared between the two groups. The correlation analyses were also performed for the cytokines concentration of aqueous humor and serum. Main Outcome Measures The concentrations of IL-1β, IL-1Ra, VEGF in aqueous humor and serum. Results Both the concentration of IL-1β (6.7±4.3 pg/ml) and IL-1Ra (657.9±445.6 pg/ml) of aqueous humor in PDR group were higher than that in control group (3.3±3.2 pg/ml and 300.8±368.0 pg/ml, both P=0.001). The concentration of IL-1β (7.6±4.6 pg/ml) and IL-1Ra (437.8±270.2 pg/ml) of serum in PDR group were also higher than that in control group (4.9±3.7 pg/ml and 279.2±226.7 pg/ml, both P=0.02). There was no significant difference for the concentration of VEGF of aqueous humor and serum between the two groups. The concentration of IL-1β of aqueous humor or serum was highly and positively correlated with the concentration of IL-1Ra of the same sample. The concentration of IL-1β (r=0.50, P=0.003) and IL-1Ra (r=0.66, P<0.001) of aqueous humor was positively correlated with that of serum, respectively, in PDR group. However, there was no correlation for concentration of VEGF of aqueous humor and serum (r=-0.06, P=0.72). Conclusion The anti-inflammation cytokine compensatively increases as the inflammation cytokine increases in both aqueous humor and serum in patients with PDR. The regulation of this inflammation response may represent a novel modality in the treatment of diabetic retinopathy. The levels of inflammation and anti-inflammation cytokines may be used as biomarkers for diabetic retinopathy. (Ophthalmol CHN, 2021, 30: 449-452)

Key words: proliferative diabetic retinopathy, VEGF, IL-1β, IL-1Ra

林仲牛彤彤杜林张晨曦张绍丹王海林. 增生性糖尿病视网膜病变患者房水及血清IL-1β与IL-1Ra含量分析[J]. 眼科, 2021, 30(6): 449-452.

Lin Zhong, Niu Tongtong, Du lin, Zhang Chenxi, Zhang Shaodan, Wang Hailin. Levels of IL-1β and IL-1Ra in aqueous humor and serum of patients with proliferative diabetic retinopathy[J]. Ophthalmology in China, 2021, 30(6): 449-452.

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增生性糖尿病视网膜病变患者房水及血清IL-1β与IL-1Ra含量分析

Levels of IL-1β and IL-1Ra in aqueous humor and serum of patients with proliferative diabetic retinopathy

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