Comparative study of short-term antiproliferative effect between suramin and taxol on trabeculetomy in rabbit

doi:10.13281/j.cnki.issn.1004-4469.2015.03.008

Abstract

Abstract: Objective To investigate the difference of short-term antiproliferative effect between taxol and suramin on trabeculetomy in rabbit. Design Experimental study. Participants 24 New Zealand albino rabbits. Methods 24 albino rabbits were randomly divided into three groups, each group consisting of 8 rabbits (16 eyes). Both eyes of rabbits were performed trabaculetomy and placed a piece of cotton with 0.3 mg/ml mitomycin C (MMC) for 2 minutes in standard control group, 0.4 mg/ml taxol for 2 minutes in taxol group, 0.4 mg/ml suramin for 2 minutes in suramin group respectively during the surgery. The filtration bleb and intraocular pressure(IOP) were observed at 3, 7, 15, and 30 days after surgery. Some conjunctival specimen were observed with hitochemicall (HE staining) and immunohistochemicall (PCNA staining) methods. Main Outcome Measures IOP, filtering bleb appearance, histopathological staining. Results The percentages of the functional blebs in taxol group and suramin group were higher than those in the control group (all P<0.05), and the percentage of the functional blebs in suramin group was higher than that in taxol group at 15 and 30 days after surgery (all P<0.05). IOPs in taxol group and suramin group were lower than that in standard control group (all P＜0.05), and IOP in suramin group was lower than that in taxol group at 7, 15, 30 days after surgery (all P＜0.05). PCNA staining showed the numbers of positive cell nuclear in taxol group and suramin group were lower than that in standard control group (all P＜0.05), and the number of PCNA staining positive cells in suramin group had been lower than that in taxol group at 7, 15, 30 days after surgery (all P＜0.05). Conclusion Taxol and suramin can maintain effectively functional blebs after trabeculectomy in rabbit. The efficacy of both 0.4 mg/ml taxol and 0.4 mg/ml suramin is better than that of 0.3mg/ml MMC, and the efficacy of suramin is the best.

Key words: text-indent: 0px, font: medium Simsun, white-space: normal, letter-spacing: normal, color: rgb(0,0,0), word-spacing: 0px, -webkit-text-stroke-width: 0px" id="x-370-anchor" name="x-370-anchor">

trabeculetomy, antiproliferative effect, taxol, suramin

YAO Bo, XIN Zhi-kun. Comparative study of short-term antiproliferative effect between suramin and taxol on trabeculetomy in rabbit [J]. Ophthalmology in China, 2015, 24(3): 167-171.

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Comparative study of short-term antiproliferative effect between suramin and taxol on trabeculetomy in rabbit

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